The single chromosome of Cunninghamia lanceolata was successfully dissected by "glass needles" under the micromanipulator in this research. The dissected Cunninghamia lanceolata chromosomes were digested by proteinase K in Eppendorf respectively. After degenerate olionucleotide primed PCR amplification (DOP PCR), DNA fragments were acquired. The suitable method of making chromosome specimens for the microdissection and PCR amplification has been discussed.